Dublin Core
Title
Development and Application of Herbal Medicine from Marine Origin
Subject
marine
Description
Chemotherapy drugs for oral cancers always cause side effects and adverse effects.
Currently natural sources and herbs are being searched for treated human oral squamous carcinoma
cells (OSCC) in an effort to alleviate the causations of agents in oral cancers chemotherapy. This
study investigates the effect of prodigiosin (PG), an alkaloid and natural red pigment as a secondary
metabolite of Serratia marcescens, to inhibit human oral squamous carcinoma cell growth; thereby,
developing a new drug for the treatment of oral cancer. In vitro cultured human OSCC models
(OECM1 and SAS cell lines) were used to test the inhibitory growth of PG via cell cytotoxic
effects (MTT assay), cell cycle analysis, and Western blotting. PG under various concentrations
and time courses were shown to effectively cause cell death and cell-cycle arrest in OECM1 and SAS
cells. Additionally, PG induced autophagic cell death in OECM1 and SAS cells by LC3-mediated
P62/LC3-I/LC3-II pathway at the in vitro level. These findings elucidate the role of PG, which may
target the autophagic cell death pathways as a potential agent in cancer therapeutics.
Keywords: prodigiosin; marine viva; autophage; oral squamous cell carcinoma
Currently natural sources and herbs are being searched for treated human oral squamous carcinoma
cells (OSCC) in an effort to alleviate the causations of agents in oral cancers chemotherapy. This
study investigates the effect of prodigiosin (PG), an alkaloid and natural red pigment as a secondary
metabolite of Serratia marcescens, to inhibit human oral squamous carcinoma cell growth; thereby,
developing a new drug for the treatment of oral cancer. In vitro cultured human OSCC models
(OECM1 and SAS cell lines) were used to test the inhibitory growth of PG via cell cytotoxic
effects (MTT assay), cell cycle analysis, and Western blotting. PG under various concentrations
and time courses were shown to effectively cause cell death and cell-cycle arrest in OECM1 and SAS
cells. Additionally, PG induced autophagic cell death in OECM1 and SAS cells by LC3-mediated
P62/LC3-I/LC3-II pathway at the in vitro level. These findings elucidate the role of PG, which may
target the autophagic cell death pathways as a potential agent in cancer therapeutics.
Keywords: prodigiosin; marine viva; autophage; oral squamous cell carcinoma
Creator
Tsong-Long Hwang;
Ping-Jyun Sung;
Chih-Chuang Liaw
Ping-Jyun Sung;
Chih-Chuang Liaw
Source
www.mdpi.com/journal/marinedrugs
Publisher
MDPI
Date
2019
Contributor
Tsong-Long Hwang;
Ping-Jyun Sung;
Chih-Chuang Liaw
Ping-Jyun Sung;
Chih-Chuang Liaw
Rights
c 2019 by the authors. Articles in this book are Open Access and distributed under the Creative
Commons Attribution (CC BY) license, which allows users to download, copy and build upon
published articles, as long as the author and publisher are properly credited, which ensures maximum
dissemination and a wider impact of our publications.
The book as a whole is distributed by MDPI under the terms and conditions of the Creative Commons
license CC BY-NC-ND.
Commons Attribution (CC BY) license, which allows users to download, copy and build upon
published articles, as long as the author and publisher are properly credited, which ensures maximum
dissemination and a wider impact of our publications.
The book as a whole is distributed by MDPI under the terms and conditions of the Creative Commons
license CC BY-NC-ND.
Relation
This is a reprint of articles from the Special Issue published online in the open access journal
Marine Drugs (ISSN 1660-3397) from 2017 to 2019 (available at: https://www.mdpi.com/journal/
marinedrugs/special issues/Herbal Medicine from Marine)
Marine Drugs (ISSN 1660-3397) from 2017 to 2019 (available at: https://www.mdpi.com/journal/
marinedrugs/special issues/Herbal Medicine from Marine)
Format
PDF
Language
ENGLISH
Type
This is a reprint of articles from the Special Issue published online in the open access journal
Marine Drugs (ISSN 1660-3397) from 2017 to 2019 (available at: https://www.mdpi.com/journal/
marinedrugs/special issues/Herbal Medicine from Marine)
Marine Drugs (ISSN 1660-3397) from 2017 to 2019 (available at: https://www.mdpi.com/journal/
marinedrugs/special issues/Herbal Medicine from Marine)